Visualizing mutational spectra using heatmaps
In my previous post, I gave a brief introduction to mutational signature and spectrum analysis using the deconstructSigs framework, including how to modify the source code to allow for the analysis of murine samples. deconstructSigs allows for the visualisation of mutational spectra as barcharts, giving a granular view of the trinucleotide context for variants in an individual sample. Unfortunately, this form of visualisation works poorly for the purpose of comparing the mutational spectra of multiple samples in a cohort. One solution to this is to display mutational spectra as a heatmap, as presented in PMK Westcott et al. 2015. This post will show how this can be done in R.
Posted: 26.11.2016